Respiratory equipment includes samples taken from patient’s nebulizers and airways clearance device (FLUTTER®, Aptalis). Samples taken from kitchen include sinks, faucets, and drains. Samples taken from bathroom include bathroom sinks, faucets, drains, and shower heads/hoses. Bar plots have been grouped according to sample type. Numeric identification on x-axis refers to patient number. Relative abundance of OTUs present in greater than 1% of samples for 34 of 39 samples collected from patients 1, 3, 5 and 6. Samples are named as follows Patient ID:S = Sputum isolate /E = environmental isolates:Strain ID number. Genotypically related isolates are highlighted in red. aeruginosa similar to two morphologically distinct isolates collected from their sputum. Current devices seem to be effective in terms of mucus expectoration and pulmonary function.

( B) The home environment of patient four (P4) also contained an isolate of P. These devices are the Positive Expiratory Pressure, the High Frequency Chest Wall Oscillation, the Oral High Frequency Oscillation, the Intrapulmonary Percussive Ventilation, the Incentive Spirometry the Flutter and the Acapella and the Cornet.

(A) The home environment of patient one (P1) contains genotypically related strains of Sphingomonas species as those found in lower airways. Genotypically related isolates found in the home environment of patients studied. with cGMPs related to our class II medical device called FLUTTER. The number of concordant isolates found in both sputum (S) and home environment (E) for patients 1–6, using culture-dependent Sanger sequencing techniques. References to Aptalis Holdings refer to Aptalis Holdings Inc. Number of morphologically distinct bacterial organisms in the home environment as a function of reservoir. Organisms that were cultured in ≥ 6% of the total cultured organisms across all patients are included.Ĭharacteristics of patients who underwent correlation of home and sputum microbiome studies. Legend shows a color representation of the taxonomic identification at the various taxonomic ranks from Sanger sequencing of the 16S rRNA DNA of the organisms. Cultured bacteria recovered from home environment represented as (Patient # 1–6) and source of isolate (S = sputum, E = environment) for all 6 patients.

Aptalis flutter device